One of the main sources of conflict was land. As English colonists sought to acquire more land for agriculture, they often came into conflict with Native American tribes who had lived on and used the land for generations. This led to a series of wars and battles, such as the Pequot War (1637) and King Philip's War (1675-1676), in which both sides suffered heavy casualties.
In addition to land conflicts, there were also cultural and religious differences that contributed to tensions between the English and Native Americans. The English viewed the Native Americans as inferior and often sought to impose their own religious and cultural practices on them. This led to resistance from Native Americans, who sought to preserve their own traditions and beliefs.
Despite these conflicts, there were also instances of cooperation and alliance between the English and Native Americans. Some Native American tribes allied with the English against their common enemies, such as the French or other rival Native American tribes. For example, the Iroquois Confederacy allied with the English during the French and Indian War (1754-1763).
Overall, the relationship between English colonists and Native Americans was characterized by both cooperation and conflict. While there were periods of peaceful coexistence and trade, there were also numerous conflicts and wars over land, resources, and cultural differences.