- Quakers: The Society of Friends, or Quakers, was founded in England in the 17th century and was a significant religious group in Pennsylvania. Quakers were known for their pacifism, simplicity, and belief in the equality of all people.
- Anglicans: The Anglican Church was the established church of England and had a presence in Pennsylvania. Anglicanism was favored by many of the colony's elites, including the Penn family, who founded Pennsylvania.
- Presbyterians: The Presbyterian Church was another Protestant denomination that was prominent in Pennsylvania. Presbyterians were known for their emphasis on education and their distinctive form of church governance.
- Lutherans: Lutheranism, a Protestant denomination originating from Germany, was practiced by many German immigrants who settled in Pennsylvania.
- German Reformed Church: The German Reformed Church was another Protestant denomination that was prevalent among German immigrants in Pennsylvania.
- Catholics: Although Catholics were often restricted in their practice in other colonies due to anti-Catholic sentiment, Pennsylvania was relatively tolerant of Catholicism, and Catholics had a presence in the colony.
- Jews: Jews were also allowed to practice their religion freely in Pennsylvania, making it a relatively inclusive colony regarding religious freedom.