- Strasbourg, the capital and largest city of the region.
- Colmar, known for its well-preserved medieval centre and traditional architecture.
- Mulhouse, a major industrial and cultural centre.
- Sélestat, a picturesque town with a rich historical heritage.
- Obernai, a charming medieval town with half-timbered houses and colourful streets.
- Kaysersberg, a charming medieval village with a well-preserved fortified city wall.
- Riquewihr, another picturesque medieval village known for its excellent wines and traditional architecture.
- Ribeauvillé, another medieval town known for its historic sites and scenic vineyards.
- Eguisheim, a beautiful medieval village with a circular layout and colourful streets.
- Munster, a spa town located in the heart of the Vosges Mountains.