- Lack of Permanent English Presence in America: The failure of Jamestown would have significantly impacted England's efforts to establish a permanent colony in North America. Without a foothold in the New World, England's colonization attempts might have been delayed or completely abandoned.
- Increased Tensions with Native Americans: The relationship between the English settlers and the Native American tribes in the region would likely have been strained if Jamestown had failed. The English colonizers' harsh treatment of and encroachment on Native American lands and resources could have led to increased hostility and conflict.
- Impact on Future English Colonization: The failure of Jamestown could have discouraged future English colonization efforts. Potential settlers might have been less inclined to venture across the Atlantic if news of Jamestown's failure and the harsh conditions in the New World spread.
- Impact on English Prestige and Economy: The failure of Jamestown would have been a significant blow to England's prestige and economic ambitions. Considerable time, effort, and resources had gone into establishing the colony, so its failure would have reflected poorly on England's ability to colonize and exploit the riches of the New World.
- Alternative European Colonization: If Jamestown failed and England decided against further colonization attempts, other European powers might have taken advantage of the opportunity. Countries such as France or Spain could have strengthened their presence in North America, potentially altering the course of European colonization in the region.