Gulf Coast:
- Florida Coast: Explored by Juan Ponce de León in 1513 and 1521.
Southeast:
- Georgia: Explored by Hernando de Soto in 1540.
Southwest:
- Arizona and New Mexico: Explored by Francisco Vásquez de Coronado in the 1540s.
West Coast:
- California Coast: Explored by Juan Rodriguez Cabrillo in the 1540s.